Flavonol synthase (FLS) is the key enzyme responsible for the biosynthesis of flavonols, the most abundant flavonoids, which have diverse pharmaceutical effects. Flavonol synthase has been previously found in other species, but not yet in Ornithogalum caudatum. See more After functional annotation, four unigenes, namely unigenes 61,710, 27,929, 76,508, and 101,341, showing high sequence similarity with flavonol synthase were retrieved from RNA-seq data (Additional file 1: … See more To determine the identity of these unigenes, nested PCRs were performed to isolate the corresponding cDNAs using O. caudatum cDNA as … See more Although OcFLS1 and OcFLS2 were grouped in the flavonol synthase family, comprehensive biochemical characterisation is … See more OcFLS1 and OcFLS2 were subcloned into pET-28a (+) to yield two recombinant constructs pET28a-OcFLS1 and pET28a-OcFLS2 … See more WebJun 1, 2024 · In this study, the partial cDNA of four genes involved in the rutin biosynthetic pathway including 4-coumaroyl CoA ligase (4CL), flavonoid 3'-hydroxylase (F3'H), flavonol synthase (FLS) and flavonol-3-O-glucoside L-rhamnosyltransferase (RT) were identified in C.spinosa plants for the first time.
Cloning and Characterization of a Flavonol Synthase Gene …
WebOct 19, 2024 · Flavonol synthase (FLS) (EC1.14.11.23) is a particularly important enzyme in the metabolism of flavonols. FLS regulates the biochemical synthesis of quercetin, … WebDec 16, 2024 · Flavonoid biosynthesis, function, and transport in plants. A Molecular structure of flavonoids, major subclasses of flavonoids, and the general flavonoid biosynthetic pathway in plants. B Biological functions of flavonoids in plants. C Intracellular and intercellular transport of flavonoids. float center rochester ny
Identification and tissue-specific expression of rutin ... - PubMed
WebNov 1, 2024 · The substrates of flavonol synthase (FLS) and dihydroflavonol reductase (DFR) were dihydroflavonol, including dihydrokaempferol (DHK), dihydroquercetin … WebIn enzymology, a flavonol synthase (EC 1.14.11.23) is an enzyme that catalyzes the following chemical reaction : dihydroflavonol + 2-oxoglutarate + O 2 ⇌ {\displaystyle … WebFLS_MALDO: 1.0e-139: 71.52: Flavonol synthase/flavanone 3-hydroxylase OS=Malus domestica GN=FLS PE=2 SV=1: FLS_EUSER: 3.9e-139: 68.06: Flavonol synthase/flavanone 3-hydroxylase OS=Eustoma exaltatum subsp. russellian... FLS_PETCR: 2.4e-136: 66.47: Flavonol synthase/flavanone 3-hydroxylase … float chair