Phosphospecific antibody
WebPhosphospecific Antibody Catalog no. 480025 (See product label for lot information) Product Description Affinity purified rabbit polyclonal antibody Clone/PAD: N/A Isotype: N/A Qty: 100μl Formulation 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol. Purification Method Page 1 of 1 Rev. 0.0 FORM-00089 WebThese phospho-specific antibodies can thus be used to track the activity of a protein, not simply its level of expression. In this review, we will discuss both the design of the phosphopeptide immunogen and immunization. The affinity purification of the phospho-specific antibody as well as the methods most suitable for characterizing the ...
Phosphospecific antibody
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WebJun 25, 2011 · Our Phosphospecific antibody microarray demonstrated changes in phosphorylation sites of some proteins after H 2 O 2 treatment. The induction of phosphorylation was approximately close to control after pretreatment with SAA. It was found that SAA affected phosphorylation proteins related to PI3K/Akt and Erk1/2 signaling. WebThis antibody is manufactured under a licensed process covered by Patent # 5, 599, 681. PI44978 (Rev 11/08) DCC-08-1089 Important Licensing Information - These products may be covered by one or more Limited Use Label Licenses (see the Invitrogen Catalog or our website, www.invitrogen.com).
WebAliquot an optimal concentration of phosphospecific fluorochrome-conjugated antibody to each tube. Incubate at room temperature for 30 minutes in the dark. Wash once with 2 mL of Stain Buffer. Centrifuge at 300g for 5–10 minutes and aspirate the supernatant. Resuspend in 500 μL of the same buffer prior to flow cytometric analysis. WebPhospho Purification Strategy: To isolate antibodies specific to the phospho epitope, serum will first be affinity purified against the non-phosphorylated peptide prior to being run through the phosphorylated peptide column. Comprehensive ELISA testing against both peptides confirms the specificity of the purified antibodies.
WebPhospho-specific antibodies, which can discriminate between the phosphorylated and non-phosphorylated forms, have emerged as demonstrated assets in the study of signal … WebSpecificity / Sensitivity. Phospho-Beclin-1 (Ser90) Antibody recognizes endogenous levels of Beclin-1 protein only when phosphorylated at Ser90. The antibody may not react with …
WebPhospho-Specific Antibodies. Protein Phosphorylation involves the addition of phosphate groups to proteins, most commonly serine (S), threonine (T) or tyrosine (Y) residue by a …
WebThis antibody is predicted to react with bovine, canine, human, mouse and non-human primate based on 100% sequence homology. In Western blot, this antibody is specific for ∽78 kDa synapsin I doublet phosphorylated at Ser549. Immunolabeling can be blocked by lambda-phosphatase treatment. the outsiders fanfiction pregnantWebPhosphoproteomics is a branch of proteomics that identifies, catalogs, and characterizes proteins containing a phosphate group as a posttranslational modification. … shura wolfWebJun 9, 2024 · Phospho-specific antibody recognizes a phosphorated form of protein at the target site. Phosphospecific antibodies are affinity-purified rabbit polyclonal or … shurayas wrath god rollWebBi-Phospho-MEK1(S218/222) Rabbit Polyclonal Antibody; Bi-MEK1抗体,Dual specificity mitogen-activated protein kinase kinase 1, MAP kinase kinase 1, MAPKK 1, MKK1, ERK activator kinase 1, MAPK/ERK kinase 1, MEK 1, MAP2K1, MEK1, PRKMK1,MAP2K1,MEK1, PRKMK1; 货号： abs106326. shura what\u0027s it gonna beWebUsing mass spectrometry and phospho-specific antibodies, we identified Ser-193 as a novel site of cytokine-induced phosphorylation within human Stat5b. Stat5b Ser(P)-193 was detected in activated primary human peripheral blood mononuc... shurayas wrath light ggWebAcetyl-CoA carboxylase (ACC) is a complex multifunctional enzyme system. ACC is a biotin-containing enzyme which catalyzes the carboxylation of acetyl-CoA to malonyl-CoA, the rate-limiting step in fatty acid synthesis. There are two ACC forms, alpha and beta, encoded by two different genes. ACC-alpha is highly enriched in lipogenic tissues. the outsiders fanfiction sisterWebMost phosphatases only dephosphorylate serine and threonine amino acids. If you are testing the phosphospecificity of a tyrosine phosphospecific antibody, lambda phosphatase is essential. The second dephosphorylation treatment is done with an alkaline phosphatase, a significantly more potent enzyme than lambda phosphatase. shurawlew igor